Separation and Purification of Two Minor Compounds from Radix isatidis by Integrative MPLC and HSCCC with Preparative HPLC
نویسندگان
چکیده
منابع مشابه
PREPARATIVE SEPARATION AND PURIFICATION OF BUFADIENOLIDES FROM ChanSu BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY COMBINED WITH PREPARATIVE HPLC
Jialian Li and Yongqing Zhang College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, 250355, P. R. China Yunliang Lin, Xiao Wang, Lei Fang* and Yanling Geng Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong, 250014, P. R. China Qinde Zhang Shandong College of Traditional Chinese Medicine, Laiyang, Shandong, 265...
متن کاملPreparative Separation of Isoflavones from Korean Soybean by HPLC
In this study, analytical and preparative HPLC systems were utilized to obtain isoflavones from Korean soybean. The mobile phases were the ternary system of water/acetonitrile/acetic acid. The optimum operating conditions were experimentally determined with the analytical column (C18, 0.46x25 cm 5 micron and the preparative column packed with 15 micron. The experimental variables were the gradi...
متن کاملAn Efficient Method for the Preparative Isolation and Purification of Flavonoids from Leaves of Crataegus pinnatifida by HSCCC and Pre-HPLC.
In this work, flavonoid fraction from the leaves of Crataegus pinnatifida was separated into its seven main constituents using a combination of HSCCC coupled with pre-HPLC. In the first step, the total flavonoid extract was subjected to HSCCC with a two-solvent system of chloroform/methanol/water/n-butanol (4:3:2:1.5, v/v), yielding four pure compounds, namely (-)-epicatechin (1), quercetin-3-O...
متن کاملSemi-preparative enantiomeric separation of ofloxacin by HPLC.
A direct semi-preparative high performance liquid chromatography (HPLC) enantioseparation of ofloxacin was performed on chemically immobilized cyclodextrin derivative-mono (6A-azido-6A-deoxy)-per(p-chlorophenyl carbamoylated) β-CD chiral stationary phase. Conditions for semi-preparative separations were established using a 250 × 4.6 mm i.d. column and subsequently extended to a 250 × 10.0 mm i....
متن کاملPreparative Purification of Corticosteroids by HPLC; Scalability and Loadability Using Agilent Prep C18 HPLC Columns Application
Agilent Prep reversed-phase columns with different diameters displayed the nearly linear scaleup for the gradient separation of corticosteroids. The loadability of the columns was quite good with as much as 8 mg of total corticosteroid injected onto a 4.6-mm column and 320 mg onto a 30-mm column.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Liquid Chromatography & Related Technologies
سال: 2014
ISSN: 1082-6076,1520-572X
DOI: 10.1080/10826076.2014.936606